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goat polyclonal primary antibody against cathepsin x  (R&D Systems)


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    R&D Systems goat polyclonal primary antibody against cathepsin x
    Cathepsin X inhibitor alleviated degeneration following intrastriatal LPS injection. (A) Representative images of methylene blue staining of coronal sections of the striatum cut at four anterior-posterior levels; between 1.68 and 1.28 mm from bregma (a,a′) ; between 0.96 and 0.56 mm from bregma (b,b′) ; between 0.00 and −0.40 mm from bregma (c,c′) ; between −0.40 and −1.40 mm from bregma (d,d′ ), 4 weeks after LPS induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS) or the presence of AMS36 (LPS/AMS36). (B) The graph shows the analysis of the size of the lateral ventricle as a sum of lateral ventricle areas in 4 anterior-posterior striatal slices at the ipsilateral side (Lesion) relative to contralateral side (Control). Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (ANOVA, Student's t -test, * p <0.5 Control vs. Lesion). (C) The graph shows the analysis of the size of striatum as a sum of striatum areas in 4 anterior-posterior striatal slices at the ipsilateral side relative to contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (Student's t -test, * p <0.5 Control vs. Lesion). (D,E) Western blot analysis of TH and iNOS expression in the contralateral (Control) and ipsilateral (Lesion) dissected striatal sections after LPS-induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS, n = 5) or the presence of AMS36 (LPS/AMS36, n = 6), using rabbit monoclonal anti-TH antibody (D) or rabbit <t>polyclonal</t> anti-iNOS antibody (E) , respectively. An antibody raised against β-actin was used as loading control. The graphs below blot shows a semi-quantitative densitometry analysis of the protein level in the ipsilateral side relative to that in the contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices of each animal were analyzed ( n = 5/6) (two-tailed Student's t -test, * p <0.05 vs. Control).
    Goat Polyclonal Primary Antibody Against Cathepsin X, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+antibody+against+x/Human%2FMouse%2FRat+Cathepsin+X%2FZ%2FP+Antibody/pmc07714997-74-18-27
    Average 94 stars, based on 26 article reviews
    goat polyclonal primary antibody against cathepsin x - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Neuroinflammation-Induced Upregulation of Glial Cathepsin X Expression and Activity in vivo"

    Article Title: Neuroinflammation-Induced Upregulation of Glial Cathepsin X Expression and Activity in vivo

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2020.575453

    Cathepsin X inhibitor alleviated degeneration following intrastriatal LPS injection. (A) Representative images of methylene blue staining of coronal sections of the striatum cut at four anterior-posterior levels; between 1.68 and 1.28 mm from bregma (a,a′) ; between 0.96 and 0.56 mm from bregma (b,b′) ; between 0.00 and −0.40 mm from bregma (c,c′) ; between −0.40 and −1.40 mm from bregma (d,d′ ), 4 weeks after LPS induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS) or the presence of AMS36 (LPS/AMS36). (B) The graph shows the analysis of the size of the lateral ventricle as a sum of lateral ventricle areas in 4 anterior-posterior striatal slices at the ipsilateral side (Lesion) relative to contralateral side (Control). Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (ANOVA, Student's t -test, * p <0.5 Control vs. Lesion). (C) The graph shows the analysis of the size of striatum as a sum of striatum areas in 4 anterior-posterior striatal slices at the ipsilateral side relative to contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (Student's t -test, * p <0.5 Control vs. Lesion). (D,E) Western blot analysis of TH and iNOS expression in the contralateral (Control) and ipsilateral (Lesion) dissected striatal sections after LPS-induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS, n = 5) or the presence of AMS36 (LPS/AMS36, n = 6), using rabbit monoclonal anti-TH antibody (D) or rabbit polyclonal anti-iNOS antibody (E) , respectively. An antibody raised against β-actin was used as loading control. The graphs below blot shows a semi-quantitative densitometry analysis of the protein level in the ipsilateral side relative to that in the contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices of each animal were analyzed ( n = 5/6) (two-tailed Student's t -test, * p <0.05 vs. Control).
    Figure Legend Snippet: Cathepsin X inhibitor alleviated degeneration following intrastriatal LPS injection. (A) Representative images of methylene blue staining of coronal sections of the striatum cut at four anterior-posterior levels; between 1.68 and 1.28 mm from bregma (a,a′) ; between 0.96 and 0.56 mm from bregma (b,b′) ; between 0.00 and −0.40 mm from bregma (c,c′) ; between −0.40 and −1.40 mm from bregma (d,d′ ), 4 weeks after LPS induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS) or the presence of AMS36 (LPS/AMS36). (B) The graph shows the analysis of the size of the lateral ventricle as a sum of lateral ventricle areas in 4 anterior-posterior striatal slices at the ipsilateral side (Lesion) relative to contralateral side (Control). Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (ANOVA, Student's t -test, * p <0.5 Control vs. Lesion). (C) The graph shows the analysis of the size of striatum as a sum of striatum areas in 4 anterior-posterior striatal slices at the ipsilateral side relative to contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (Student's t -test, * p <0.5 Control vs. Lesion). (D,E) Western blot analysis of TH and iNOS expression in the contralateral (Control) and ipsilateral (Lesion) dissected striatal sections after LPS-induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS, n = 5) or the presence of AMS36 (LPS/AMS36, n = 6), using rabbit monoclonal anti-TH antibody (D) or rabbit polyclonal anti-iNOS antibody (E) , respectively. An antibody raised against β-actin was used as loading control. The graphs below blot shows a semi-quantitative densitometry analysis of the protein level in the ipsilateral side relative to that in the contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices of each animal were analyzed ( n = 5/6) (two-tailed Student's t -test, * p <0.05 vs. Control).

    Techniques Used: Injection, Staining, Control, Western Blot, Expressing, Two Tailed Test

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    Cathepsin X inhibitor alleviated degeneration following intrastriatal LPS injection. (A) Representative images of methylene blue staining of coronal sections of the striatum cut at four anterior-posterior levels; between 1.68 and 1.28 mm from bregma (a,a′) ; between 0.96 and 0.56 mm from bregma (b,b′) ; between 0.00 and −0.40 mm from bregma (c,c′) ; between −0.40 and −1.40 mm from bregma (d,d′ ), 4 weeks after LPS induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS) or the presence of AMS36 (LPS/AMS36). (B) The graph shows the analysis of the size of the lateral ventricle as a sum of lateral ventricle areas in 4 anterior-posterior striatal slices at the ipsilateral side (Lesion) relative to contralateral side (Control). Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (ANOVA, Student's t -test, * p <0.5 Control vs. Lesion). (C) The graph shows the analysis of the size of striatum as a sum of striatum areas in 4 anterior-posterior striatal slices at the ipsilateral side relative to contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (Student's t -test, * p <0.5 Control vs. Lesion). (D,E) Western blot analysis of TH and iNOS expression in the contralateral (Control) and ipsilateral (Lesion) dissected striatal sections after LPS-induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS, n = 5) or the presence of AMS36 (LPS/AMS36, n = 6), using rabbit monoclonal anti-TH antibody (D) or rabbit <t>polyclonal</t> anti-iNOS antibody (E) , respectively. An antibody raised against β-actin was used as loading control. The graphs below blot shows a semi-quantitative densitometry analysis of the protein level in the ipsilateral side relative to that in the contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices of each animal were analyzed ( n = 5/6) (two-tailed Student's t -test, * p <0.05 vs. Control).
    Goat Polyclonal Antibody Against Human X, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cathepsin X inhibitor alleviated degeneration following intrastriatal LPS injection. (A) Representative images of methylene blue staining of coronal sections of the striatum cut at four anterior-posterior levels; between 1.68 and 1.28 mm from bregma (a,a′) ; between 0.96 and 0.56 mm from bregma (b,b′) ; between 0.00 and −0.40 mm from bregma (c,c′) ; between −0.40 and −1.40 mm from bregma (d,d′ ), 4 weeks after LPS induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS) or the presence of AMS36 (LPS/AMS36). (B) The graph shows the analysis of the size of the lateral ventricle as a sum of lateral ventricle areas in 4 anterior-posterior striatal slices at the ipsilateral side (Lesion) relative to contralateral side (Control). Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (ANOVA, Student's t -test, * p <0.5 Control vs. Lesion). (C) The graph shows the analysis of the size of striatum as a sum of striatum areas in 4 anterior-posterior striatal slices at the ipsilateral side relative to contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (Student's t -test, * p <0.5 Control vs. Lesion). (D,E) Western blot analysis of TH and iNOS expression in the contralateral (Control) and ipsilateral (Lesion) dissected striatal sections after LPS-induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS, n = 5) or the presence of AMS36 (LPS/AMS36, n = 6), using rabbit monoclonal anti-TH antibody (D) or rabbit <t>polyclonal</t> anti-iNOS antibody (E) , respectively. An antibody raised against β-actin was used as loading control. The graphs below blot shows a semi-quantitative densitometry analysis of the protein level in the ipsilateral side relative to that in the contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices of each animal were analyzed ( n = 5/6) (two-tailed Student's t -test, * p <0.05 vs. Control).
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    Cathepsin X inhibitor alleviated degeneration following intrastriatal LPS injection. (A) Representative images of methylene blue staining of coronal sections of the striatum cut at four anterior-posterior levels; between 1.68 and 1.28 mm from bregma (a,a′) ; between 0.96 and 0.56 mm from bregma (b,b′) ; between 0.00 and −0.40 mm from bregma (c,c′) ; between −0.40 and −1.40 mm from bregma (d,d′ ), 4 weeks after LPS induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS) or the presence of AMS36 (LPS/AMS36). (B) The graph shows the analysis of the size of the lateral ventricle as a sum of lateral ventricle areas in 4 anterior-posterior striatal slices at the ipsilateral side (Lesion) relative to contralateral side (Control). Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (ANOVA, Student's t -test, * p <0.5 Control vs. Lesion). (C) The graph shows the analysis of the size of striatum as a sum of striatum areas in 4 anterior-posterior striatal slices at the ipsilateral side relative to contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (Student's t -test, * p <0.5 Control vs. Lesion). (D,E) Western blot analysis of TH and iNOS expression in the contralateral (Control) and ipsilateral (Lesion) dissected striatal sections after LPS-induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS, n = 5) or the presence of AMS36 (LPS/AMS36, n = 6), using rabbit monoclonal anti-TH antibody (D) or rabbit <t>polyclonal</t> anti-iNOS antibody (E) , respectively. An antibody raised against β-actin was used as loading control. The graphs below blot shows a semi-quantitative densitometry analysis of the protein level in the ipsilateral side relative to that in the contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices of each animal were analyzed ( n = 5/6) (two-tailed Student's t -test, * p <0.05 vs. Control).
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    Cathepsin X inhibitor alleviated degeneration following intrastriatal LPS injection. (A) Representative images of methylene blue staining of coronal sections of the striatum cut at four anterior-posterior levels; between 1.68 and 1.28 mm from bregma (a,a′) ; between 0.96 and 0.56 mm from bregma (b,b′) ; between 0.00 and −0.40 mm from bregma (c,c′) ; between −0.40 and −1.40 mm from bregma (d,d′ ), 4 weeks after LPS induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS) or the presence of AMS36 (LPS/AMS36). (B) The graph shows the analysis of the size of the lateral ventricle as a sum of lateral ventricle areas in 4 anterior-posterior striatal slices at the ipsilateral side (Lesion) relative to contralateral side (Control). Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (ANOVA, Student's t -test, * p <0.5 Control vs. Lesion). (C) The graph shows the analysis of the size of striatum as a sum of striatum areas in 4 anterior-posterior striatal slices at the ipsilateral side relative to contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (Student's t -test, * p <0.5 Control vs. Lesion). (D,E) Western blot analysis of TH and iNOS expression in the contralateral (Control) and ipsilateral (Lesion) dissected striatal sections after LPS-induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS, n = 5) or the presence of AMS36 (LPS/AMS36, n = 6), using rabbit monoclonal anti-TH antibody (D) or rabbit <t>polyclonal</t> anti-iNOS antibody (E) , respectively. An antibody raised against β-actin was used as loading control. The graphs below blot shows a semi-quantitative densitometry analysis of the protein level in the ipsilateral side relative to that in the contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices of each animal were analyzed ( n = 5/6) (two-tailed Student's t -test, * p <0.05 vs. Control).
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    Image Search Results


    Cathepsin X inhibitor alleviated degeneration following intrastriatal LPS injection. (A) Representative images of methylene blue staining of coronal sections of the striatum cut at four anterior-posterior levels; between 1.68 and 1.28 mm from bregma (a,a′) ; between 0.96 and 0.56 mm from bregma (b,b′) ; between 0.00 and −0.40 mm from bregma (c,c′) ; between −0.40 and −1.40 mm from bregma (d,d′ ), 4 weeks after LPS induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS) or the presence of AMS36 (LPS/AMS36). (B) The graph shows the analysis of the size of the lateral ventricle as a sum of lateral ventricle areas in 4 anterior-posterior striatal slices at the ipsilateral side (Lesion) relative to contralateral side (Control). Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (ANOVA, Student's t -test, * p <0.5 Control vs. Lesion). (C) The graph shows the analysis of the size of striatum as a sum of striatum areas in 4 anterior-posterior striatal slices at the ipsilateral side relative to contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (Student's t -test, * p <0.5 Control vs. Lesion). (D,E) Western blot analysis of TH and iNOS expression in the contralateral (Control) and ipsilateral (Lesion) dissected striatal sections after LPS-induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS, n = 5) or the presence of AMS36 (LPS/AMS36, n = 6), using rabbit monoclonal anti-TH antibody (D) or rabbit polyclonal anti-iNOS antibody (E) , respectively. An antibody raised against β-actin was used as loading control. The graphs below blot shows a semi-quantitative densitometry analysis of the protein level in the ipsilateral side relative to that in the contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices of each animal were analyzed ( n = 5/6) (two-tailed Student's t -test, * p <0.05 vs. Control).

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Neuroinflammation-Induced Upregulation of Glial Cathepsin X Expression and Activity in vivo

    doi: 10.3389/fnmol.2020.575453

    Figure Lengend Snippet: Cathepsin X inhibitor alleviated degeneration following intrastriatal LPS injection. (A) Representative images of methylene blue staining of coronal sections of the striatum cut at four anterior-posterior levels; between 1.68 and 1.28 mm from bregma (a,a′) ; between 0.96 and 0.56 mm from bregma (b,b′) ; between 0.00 and −0.40 mm from bregma (c,c′) ; between −0.40 and −1.40 mm from bregma (d,d′ ), 4 weeks after LPS induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS) or the presence of AMS36 (LPS/AMS36). (B) The graph shows the analysis of the size of the lateral ventricle as a sum of lateral ventricle areas in 4 anterior-posterior striatal slices at the ipsilateral side (Lesion) relative to contralateral side (Control). Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (ANOVA, Student's t -test, * p <0.5 Control vs. Lesion). (C) The graph shows the analysis of the size of striatum as a sum of striatum areas in 4 anterior-posterior striatal slices at the ipsilateral side relative to contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices from each animal were analyzed ( n = 5/6) (Student's t -test, * p <0.5 Control vs. Lesion). (D,E) Western blot analysis of TH and iNOS expression in the contralateral (Control) and ipsilateral (Lesion) dissected striatal sections after LPS-induced lesion in the absence of cathepsin X inhibitor AMS36 (LPS, n = 5) or the presence of AMS36 (LPS/AMS36, n = 6), using rabbit monoclonal anti-TH antibody (D) or rabbit polyclonal anti-iNOS antibody (E) , respectively. An antibody raised against β-actin was used as loading control. The graphs below blot shows a semi-quantitative densitometry analysis of the protein level in the ipsilateral side relative to that in the contralateral side. Values are means ± SD of group of 5 animals for LPS ( n = 5) and group of 6 animals for LPS/AMS36 ( n = 6), where 4 anterior-posterior striatal slices of each animal were analyzed ( n = 5/6) (two-tailed Student's t -test, * p <0.05 vs. Control).

    Article Snippet: They were then incubated overnight at 4°C with mouse monoclonal antibody against TH (1:750, Abcam, Cambridge, UK) or goat polyclonal primary antibody against cathepsin X (1:200, AF934, R&D Systems, MN, United States), diluted in blocking solution.

    Techniques: Injection, Staining, Control, Western Blot, Expressing, Two Tailed Test